The most emotional moment of the Mass Effect Trilogy?

When my canon Shepard (who romanced Ashley) went to visit her at the Huerta Memorial Hospital after she gets beaten by Eva Coré. The first two visits were the most emotional in my opinion. Also the goodbye scene for her before running to the beam on Earth.

I found leaving Earth and watching that the shuttle that kid was in be destroyed by the Reapers to be very emotional, particularly the first time playing and not knowing about what that kid represented (assuming the kid wasn't real, and there's evidence he might not be).

Conrad sacrificing his life to prevent Shepard from being killed, if Jenna's mission in ME1 is not completed

Couldn't pick one, even one from each game.
ME1- I Remember Me
ME1- Saving the Council
ME2- 'Mommy loves you very much!'
ME2- Entire suicide mission
ME3- Tali's suicide (avoided spoilers, had no idea it was possible)
ME3- Samara's suicide (avoided spoilers, had no idea it was possible)
ME3- shooting Mordin (it was the right call, I don't care what anyone says)
ME3- end of Citadel DLC, 'The best.'.
ME3- Femshep 'What do you need me to do?'

Many more.

ME3 dominates but that's because it takes much loved characters and, on the whole, gives them closure in a fitting manner, no matter what decision you made. It might be the weakest game but has some of the best character moments. Of course, they've been earned.

This is more nostalgic than emotional, but I just love to see those characters from ME1 to meet you in further games. Gianna Parasini and Captain Kirrahe especially (also Elisabeth? from Exogeni corp.!). It reminded me that Shepard has a support everywhere and has made an impact with her career.

My most f*cked up and very depressing moment in the games is the destruction of the Batarian system. When Shepard watches helplessly from the distance, and when Hackett visits Shep in the Cerberus vessel afterwards.

"I don't need your reports to know you did the right thing."

Everything to do with EDI in the Destroy ending was pretty gut-wrenching. Seeing her face on one of the slides and her name on the memorial wall, but especially watching Joker trying to outrun the energy wave - he doesn't even know.

Samara's mission in ME2.
Samara killing her own daughter and loosing her cool.

I also always shed a tear for Miranda's sister. I think preventing Miranda to greet her sister after the mission is the most pure evil thing to do in the whole series. Seeing Miranda cry.

The loyalty missions in ME2 were rad.

The Goodbye scene in London always makes me cry no matter how many times ive played it, no matter how many times i romance the same character, and no matter how much ive memorized each and every line... It still breaks me everytime

Curing the genophage in ME3 and Rannoch arc.

There is a line in ME2 that always gets me during Tali's trial, if you get charges dropped:

Shala'Raan: Commander Shepard, please accept these gifts for taking the time to represent one of our people.
Shepard: With all due respect Admiral, I didn't represent one of your people. I represented one of mine.

I thought no one would mention any of mine; but someone actually did mention one.

I have three moments that I will tell, in order:

1 - Meeting sovereign in ME1. I find it curious I'm the only one to mention it. For me it was both amazing and terrifying. In that moment, we knew nothing of the reapers. They sounded like an unfathomable lovecraftian horror, and actually were more impressive than the "team kill everyone to save everyone obey the starchildren" suckers they eventually became. I remember the deep voice, saying: "We are each a nation, free of all weakness"; "your lifes are measured in years and decades; you wither and die; We are eternal", and "you meddle in matters you can't know; fumbling in ignorance". I'd have loved that they kept the cosmic horror aspect alive all the way to the end.

2 - the very endgame of ME2; specifically, the scene when Harbinger leaves the collector general for the last time, just before we blow the collector's base. What gets me is the stupid insectoid absent gaze the collector shows when he is left just before being blown to pieces. That single moment entirely signaled, to me, the fall of the previous cicle; the death of the empire, and the depths of degradation imposed by the reapers, and made the fate of the protheans so very sad...

3 - the end of arrival. You sacrifice thousands and its justified because you save trillions. You are still a mass murderer, justified as it is. I guess I just can't be cool with it.

Regards .

Killing Wrex on Virmire. The full voice acting, the cinematic story-telling, it was all fresh and new at the time. Add to that that this is the first time I've had the option to kill a major character in an RPG not because I wanted to be EVIIIL, but because it had to be done, and that was a hell of a punch.

There are so many moments in the trilogy and most people have nailed them here.
I want to highlight an emotional one for me - choosing to either rewrite or destroy the Geth Heretics. I hated both outcomes.

It didn't make me laugh or cry, but I was all up in my emotions saying to myself, "what did I just do?"