What was your most "killed off characters" playthrough?

This is like the thread on BSN, except it was titled, 'A Lonely Playthrough' created by Excella Gionne

Basically just post your playthrough of the characters you had die whether they were squadmates or not.

This is a playthrough I did about 2 years ago

Xenia Shepard - Earthborn/ruthless/engineer

ME1

let Toombs kill the scientist and he will kill himself
when helping Dr. Michel with her problem, kill the blackmailer
when helping detective Chellick, kill Jax and his 2 thugs. This will have Jenna show up in ME3 to save Conrad if he is alive
kill Helena Blake
kill father Kyle and his followers
kill Darius during the UNC Negotiation mission
before killing Fist, kill the 2 workers
talk with Conrad so he shows up in ME2
when scanning the keepers kill Chorban, Here's how
on Feros kill all the colonists and Shiala
kill Jeong
kill Dr. Ross and the 3 mercs during the exogeni facility mission
Noveria--have Anoleis and Gianna Parasini kill each other. Here's the video to do this. Kill the Rachni Queen
When going to Lorik Qui'in's office, kill the two guards instead of letting them leave
kill Inamorda and the 2 krogan thugs during the Noveria smuggling mission
let the leader kill chairman burns and kill the leader and thugs
On Virmire, kill Rana Thanopolis and let Kirrahe and Kaidan die. Keep Wrex and Ashley alive. Also kill the salarian prisoners
kill Charn and then Kill Balak in Bring Down the Sky dlc causing the hostages to die
let the council die

ME2

On Omega make the Batarian drink his poison. Let the Patriarch die fighting the killers. When going to recruit archangel, let the kid join the mercs only to watch him be killed by Garrus a few minutes later.
On Mordin's recruitment misson, kill the 3 district guards, don't give medigel to the Batarian and when you encounter Mordin's assistant, kill the Batarians who will kill the assistant
N7 mission Javelin Missle Launcher. Choose to save the spaceport and let the people die
talk with Conrad or tell him to get lost and he will die
let Jack kill Aresh on her loyalty mission
kill the 2 krogan bodyguards and let Garrus kill Sidonis on his loyalty mission
let the girl get killed and have Jacob's father die at the hands of his crew
on Tali's recruitment mission, take your time destroying the Colossus, Kal'Reeger will die
kill the krogan spokesman
kill the sick Krogan in Mordin's loyalty mission
let Mordin kill Maelon and save the data
On Samara's recruitment mission kill Elnora
let Niftu Cal, biotic god, die
On Thane's recruitment mission, don't give medigel to the salarian, push the merc out the window and on his loyalty mission kill Joram Talid
Jack, Kasumi and Thane killed because of no upgrades
Garrus dies being sent in the vent
Jacob was carried off by the swarmers
Sent no escort for the crew. They will die
Grunt killed as 2nd fire team leader
Miranda and Tali killed fighting the terminator
Mordin killed holding the line
After the suicide mission, kill Zaeed during his loyalty mission
Samara and Legion live to be in ME3
Do the Arrival dlc to kill 300,000 Batarians

ME3

don't bring Allers on the ship, she will die onboard the SSV Shasta
have General Oraka killed
save Jondam Bau, the salarian spectre, letting the Hanar lose their homeworld
don't talk to the female student on Grissom. She will die from her wounds
Ensign Prangley is killed because Jack died during the suicide mission
Conrad Verner dies because Jenna isn't there to help if her mission wasn't done in ME1
Sabotage the genophage by shooting Paddok Wiks in the back. Don't do Turian Bomb causing Eve to die with other Krogan. Later on kill Wrex on the Citadel.
overload the reactor killing thousands on Omega. Kill Oleg Petrovsky
the Salarian Councillor dies because Kirrahe and Thane are dead
let Captain Aaron Summers kill the prisoner
pick the geth. The Quarians will die. Legion dies uploading the code.
let Anne Bryson die
shoot Brooks in Citadel dlc
during the N7 fuel reactor mission, do not send help to Captain Riley. She will die
let Samara kill herself and then kill Falere
let Oriana(Miranda's sister) and father die. Here's how to.
don't talk to Ashley when she's in the hospital, then shoot her and she will say "she hopes the reapers send you to he**". Here's the video. If you want to make it more interesting kill her while wearing Cerberus armor.
after saving the Volus Ambassador take the Volus fleet and ignore the Turian colony
don't talk to Steve, he will die in London
don't do the From Ashes dlc
let TIM kill Anderson
pick destroy causing edi to fry along with the Geth

At the memorial scene I had Samantha, Joker, Liara and James in attendance. I had over 3100 ems, so femshep isn't dead. On the wall I had both columns filled with names(48) and Anderson in the middle(49) and Kasuni and Zaeed were dead and their names weren't on the wall giving me 51 dead

I did a new game plus with the same charcter, but I went for low ems. James and Liara were killed on the beam run(ems 1750-2000) and femshep dead when picking destroy. The memoral scene only had Samantha and Joker present. On the wall I had 50 names(including femshep) and 4 names not on the wall giving me 54 dead

If your ems is below 1750 the doors to the Normandy don't open.

I estimate that 17 315 105 died plus all the random mercs that were killed throughout the trilogy